![]() ![]() b Protein expression of Pax7 and MyoD (upper band) transcription factors in SC cocultured with/without ADSC in proliferation-induction medium. a Relative mRNA level of paired box protein domain 7 (Pax7) and myogenic differentiation antigen (MyoD) transcription factors in SC cocultured with/without ADSC in proliferation-induction medium. Cellular therapy with ADSC represents a novel approach to promote skeletal muscle regeneration.Īdipose-derived stem cells Crush injury Skeletal muscle regeneration Stem cell therapy Tissue engineering.Įffect of adipose-derived stem cells (ADSC) on satellite cell (SC) activation and differentiation. Additionally, an increase in fiber cross-sectional size and in the number of mature fibers with centralized nuclei was observed.ĪDSC transplantation into acute damaged skeletal muscle significantly improves functional muscle tissue regeneration without direct participation in muscle fiber formation. However, the ADSC-injected muscles had improved contractility in comparison with the collagen-injected controls 28 days post-transplantation. We did not observe ADSC differentiation into new muscle fibers or endothelial cells. Intramuscular transplantation of ADSC into crushed tibialis anterior muscle leads to an improved muscle regeneration with ADSC residing in the damaged area. The muscle contractility was assessed by myography in an organ bath system. Recovering muscles were analyzed for gene and protein expression by real-time quantitative polymerase chain reaction and immunohistochemistry. Mouse ADSC were isolated from subcutaneous fat tissue and transplanted with a collagen hydrogel into the crushed tibialis anterior muscle of mice. The aim of this study was to evaluate the effect of ADSC transplantation on functional skeletal muscle regeneration in an acute injury model. Adipose-derived stem cells (ADSC) are a highly abundant source of progenitor cells that have been previously reported to support the regeneration of various muscle tissues, including striated muscles. However, extensive damage that exceeds the self-regenerative ability of the muscle can lead to irreversible fibrosis, scarring, and significant loss of function. muscle has a remarkable regenerative capacity. 7 University Clinic for Visceral Surgery and Medicine, Bauchzentrum Bern, Inselspital, CH-3010, Bern, Switzerland. 6 Department of Clinical Research, Laboratory for Visceral Surgery and Medicine, University of Bern, Murtenstrasse 35, 3008, Bern, Switzerland.5 University Clinic for Visceral Surgery and Medicine, Bauchzentrum Bern, Inselspital, CH-3010, Bern, Switzerland.4 Current address: Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.3 Theodor Kocher Institute, University of Bern, Bern, Switzerland.2 Department of Urology, Laboratory for Tissue Engineering and Stem Cell Therapy, University Hospital Zurich, Zurich, Switzerland. ![]() 1 Department of Clinical Research, Laboratory for Visceral Surgery and Medicine, University of Bern, Murtenstrasse 35, 3008, Bern, Switzerland.
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